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(A). All of the Aspc1, Bxpc3 and Panc1 cells formed spheres in serum-free DMEM/F-12 containing bFGF, EGF, B27 and insulin. (B, C and D). The sphere cells of Aspc1, Bxpc3 and Panc1 strongly expressed CD44, CD24, ESA, CD133 and nestin after they had been cultured for more than 96 hours in serum-free DMEM/F-12 containing bFGF, EGF, B27 and insulin.
