Table 1.
Gene | Primer | Sequence | Size(bp) |
---|---|---|---|
Gli1 | Forward | 5′ TGGGCAACATGAGTGTCTTACTG 3′ | 93 |
Reverse | 5′ TGTGATGGATGAGATTCCCTACT 3′ | ||
Notch1 | Forward | 5′ CCTGCCCGTTCTTGAAATGT 3′ | 186 |
Reverse | 5′ GCATCTTCTTCGGAACCTGG 3′ | ||
β-catenin | Forward | 5′ AAATAAGCAGGTGGATCTATTTCAT 3 | 116 |
Reverse | 5′ ACTGGTAAACTGTCCAAAACAAGGT 3′ | ||
Oct4 | Forward | 5′ AAGGGCAAGCGATCAAGCA 3′ | 97 |
Reverse | 5′ GGGCCAGAGGAAAGGACACT 3′ | ||
β-actin | Forward | 5′CCTCTCCCAAGTCCACACAG 3′ | 130 |
Reverse | 5′GGGCACGAAGGCTCATCATT 3′ |