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. 2015 Dec 22;15(3):455–470. doi: 10.1080/15384101.2015.1127478

Figure 7.

Figure 7.

Activation of p53 and induction of p53-dependent apoptosis by ARTIK-52 in sensitive but not resistant cells. A-C. Western blotting of total cell extracts of ARTIK-52 sensitive, CWR22R (A) and MCF7 (B) cells, or resistant, RCC45 cells (C), treated with ARTIK-52 or CBL0137 (in μM) for 16 hours. * - reduction of p53 level in MCF7 cells in response to 1μM of CBL0137 is due to high toxicity of CBL0137 for these cells. D. Caspase 3/7 activation in p53 wild type and mutant cells in response to treatment with ARTIK-52 (1 μM) or doxorubicin (1 μM) for 16 hours. Bars correspond to fold changed compared with untreated control. Error bars – standard deviation between 3 replicates within one experiment. E. Effect of p53 inactivation by dominant negative mutant GSE56 on ARTIK-52 induced reduction of AR level in CWR22R cells. Western blotting of extracts of cells transduced with either GSE56 or empty vector and treated with indicated concentrations of ARTIK-52 for 16 hours. F. ARTIK-52 treatment causes phosphorylation of serine 15 of p53 in sensitive cells. Western blotting of extracts of ARTIK-52 sensitive (MCF7 and CWR22R) or resistant (NKE) cells treated with 1 μM of ARTIK-52 during indicated periods of time.