Figure 2.

50 Hz ELF-EMF exposure does not alter the unmethylated status of the miR-26b-5p host gene CTDSP1. (A) Schematic of the CpG islands in the transcriptional region of CTDSP1. CpG sites are indicated by vertical orange lines. The regions of MSP are indicated. (B) CTDSP1 was unmethylated in GC-2 cells at different magnetic field intensities of ELF-EMF exposure. Each lane contains products that were amplified from separate PCRs using primers that were specific for methylated (M) or unmethylated (U) DNA templates. PC: positive control. NC: negative control.