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. 2015 Dec 4;15(3):357–367. doi: 10.1080/15384101.2015.1120924

Figure 5.

Figure 5.

CCND2 is a direct target of miR-26b-5p. (A) Prediction results using 3 bioinformatics algorithms (TargetScan, PicTar and PITA). We identified USP25, PRKCD, FNIP1, EZH2 and CCND2 as potential target genes for further investigation. (B) The mRNA expression of potential target genes was measured by qRT-PCR. The mRNA expression showed a negative correlation between miR-26b-5p and the potential target genes. (C) CCND2 protein expression was analyzed using Western blotting. MiR-26b-5p can regulate the protein levels of CCND2. (D) The schematic representation of the binding sites for miR-26b-5p in the 3’-UTR of CCND2 using TargetScan software.