Figure 2.
Effect of MLC2 KD on mouse oocyte maturation. Fully grown oocytes microinjected with MLC2-MO were arrested at GV stage with milrinone for 22–24 h to block mRNA translation, washed in milrinone-free medium, and then cultured for 12 h to examine the rate of polar body extrusion. (A) p-MLC2 expression was significantly reduced after MLC2 morpholino injection by protein gel blot. (B) Polar body extrusion failure after MLC2 morpholino injection. The rate of polar body extrusion was decreased after MLC2 morpholino injection or antibody (Ab) injection. Images were acquired with a camera on a stereomicroscope. Arrows showed that the control oocytes extruded the polar body while the treated oocytes failed. *, significant difference (p < 0.05).