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. 2016 Jul 13;10(7):e0004771. doi: 10.1371/journal.pntd.0004771

Fig 2. Multiple sequence alignment and molecular phylogenetic analysis of the sand fly RGD protein family.

Fig 2

(A) Alignment of the LolRGD and the LolnoRGD identified from the B. olmeca salivary gland transcriptome. Black background shading represents identical amino acids. Grey background shading represents similar amino acids. Yellow background shading highlights the RGD motif. (B) The RGD protein phylogeny depicts three distinct branched, one encompassing N. intermedia sequences, another one including Lu. longipalpis and Lu. ayacuchensis proteins, and a third clade unique to B. olmeca, which includes the gene copy devoid of the RGD motif as well as the one containing such a motif. The evolutionary history was inferred based on the Whelan And Goldman model [62]. Sand fly species are indicated by the different symbols in the legend on the right.