Table 1. Bone phenotype of Control mice (Ctrl) as compared to β-catenin conditional knock-out heterozygous mice (cKO HET) at 14 weeks (in vivo) and 19 weeks (ex vivo).
| Age | Position | Parameter | Female | Male | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ctrl | HET | Ctrl | HET | |||||||
| mean±SD | N | mean±SD | N | mean±SD | N | mean±SD | N | |||
| 14 weeks | Diaphyseal Cortical | BMD (mg HA/ccm) | 1060.11±13.90 | 6 | 1065.87±25.19 | 10 | 1058.99±12.79 | 14 | 1071.53±16.28 | 6 |
| BV/TV (%) | 88.89±1.14 | 6 | 86.99±1.66* | 10 | 90.08±1.74 | 14 | 89.78±2.56 | 6 | ||
| Ct.Th (mm) | 0.21±0.01 | 6 | 0.20±0.01* | 10 | 0.23±0.02 | 14 | 0.24±0.01 | 6 | ||
| Metaphyseal Cortical | BMD (mg HA/ccm) | 925.98±22.37 | 8 | 933.6±29.77 | 17 | 925.5±14.69 | 14 | 938.56±20.45 | 13 | |
| BV/TV (%) | 75.44±2.77 | 8 | 75.18±6.09 | 17 | 76.32±3.70 | 14 | 78.68±2.57 | 13 | ||
| Ct.Th (mm) | 0.12±0.01 | 8 | 0.12±0.01 | 17 | 0.12±0.01 | 14 | 0.13±0.01 | 13 | ||
| Metaphyseal Trabecular | BMD (mg HA/ccm) | 753.36±17.82 | 9 | 757.29±27.13 | 17 | 730.53±18 | 14 | 720.05±25.64 | 14 | |
| BV/TV (%) | 8.71±1.47 | 9 | 8.12±2.10 | 17 | 19.42±4.53 | 14 | 19.93±1.76 | 14 | ||
| Tb.N (1/mm) | 2.13±0.24 | 9 | 2.00±0.34 | 17 | 4.51±0.41 | 14 | 3.73±0.52* | 14 | ||
| Tb.Th (mm) | 0.06±0.00 | 9 | 0.06±0.01 | 17 | 0.07±0.01 | 14 | 0.07±0.00 | 14 | ||
| Tb.Sp (mm) | 0.49±0.06 | 9 | 0.53±0.08 | 17 | 0.23±0.02 | 14 | 0.29±0.05* | 14 | ||
| 19 weeks | Diaphyseal Cortical | BMD (mg HA/ccm) | 1196.14±27.44 | 7 | 1169.43±24.24 | 6 | 1154.87±43.20 | 6 | 1149.55±32.64 | 6 |
| BV/TV (%) | 91.32±0.51 | 7 | 90.47±0.34* | 6 | 89.27±4.84 | 6 | 90.45±0.72 | 6 | ||
| Ct.Th (mm) | 0.21±0.01 | 7 | 0.19±0.01* | 6 | 0.21±0.01 | 6 | 0.19±0.01* | 6 | ||
| Metaphyseal Cortical | BMD (mg HA/ccm) | 1053.11±24.27 | 7 | 1047.59±42.81 | 6 | 1025.03±27.98 | 6 | 1013.88±27.52 | 6 | |
| BV/TV (%) | 85.40±1.62 | 7 | 84.54±1.68 | 6 | 81.97±1.82 | 6 | 80.48±7.39 | 6 | ||
| Ct.Th (mm) | 0.13±0.01 | 7 | 0.12±0.01 | 6 | 0.10±0.01 | 6 | 0.10±0.01 | 6 | ||
| Metaphyseal Trabecular | BMD (mg HA/ccm) | 913.24±30.76 | 7 | 916.49±54.78 | 6 | 877.35±15.46 | 6 | 877.87±25.64 | 6 | |
| BV/TV (%) | 8.95±1.29 | 7 | 7.71±2.34 | 6 | 13.96±2.71 | 6 | 8.92±0.95* | 6 | ||
| Tb.N (1/mm) | 2.65±0.29 | 7 | 2.54±0.53 | 6 | 4.88±0.51 | 6 | 3.95±0.44* | 6 | ||
| Tb.Th (mm) | 0.06±0.00 | 7 | 0.05±0.00 | 6 | 0.05±0.00 | 6 | 0.04±0.00* | 6 | ||
| Tb.Sp (mm) | 0.39±0.05 | 7 | 0.41±0.08 | 6 | 0.20±0.02 | 6 | 0.26±0.03* | 6 | ||
Cortical bone mineral density (BMD), bone volume (BV/TV) and cortical thickness (Ct.Th) were analyzed at the diaphysis and at the metaphysis as shown in the Fig 2. Trabecular bone mineral density (BMD), bone volume (BV/TV), number (Tb.N), thickness (Tb.Th) and spacing (Tb.Sp) were analyzed at the metaphysis. N = number of animals per group.
*: represents a significant difference between Control and HET mice (p<0.05). Independent sample t test was used to compare the effect of HET deletion in the NH groups (HET versus controls). Data represents mean ± SD.