Figure 2.
Inhibition of CPT1 led to decreased number of primordial germ cells (PGCs). (A) PGC number in E14.5 genital ridges cultured in normal medium containing various concentrations of etomoxir estimated by detection of DDX4-positive PGCs immunofluorescence staining. Bar =50 μm. (B) Average number of DDX4-positive PGCs in E14.5 genital ridge per 105 μm2. PGCs were counted in 3 sections from the middle part of individual genital ridges over the course of 3 independent experiments. Number of PGCs progressively decreased with inhibition of CPT1. (**), P < 0.01.