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Verification of p53 activation dependent G1 arrest. (A) Western blot analysis showed expression of P-p53 and p21 increased with inhibition of fatty acid degradation. ACTIN was used as a control. (B and C). Quantity of PGCs was determined. Compared with controls, total number of PGCs significantly decreased after addition of CPT1 inhibitor. Total number of PGCs was restored to control levels by simultaneous addtion of CPT1 and p53 inhibitors into normal medium.
