TABLE II.

Primer Sequences and Product Sizes for Quantitative Reverse Transcription-Polymerase Chain Reaction

Gene		5′ DNA sequence 3′	Product size (bp)
Collagen I	Forward	5′ GTCACCCACCGACCAAGAAACC 3′	121
	Reverse	5′ AAGTCCAGGCTGTCCAGGGATG 3′
Collagen III	Forward	5′ GCCAACGTCCACACCAAATT 3′	88
	Reverse	5′ AACACGCAAGGCTGTGAGACT 3′
Tenomodulin	Forward	5′ TGTATTGGATCAATCCCACTCTAAT 3′	92
	Reverse	5′ TTTTTCGTTGGCAGGAAAGT 3′
Tenascin C	Forward	5′ TGGGCAGATTTCACGGCTG 3′	207
	Reverse	5′ TGCTCTGAGCCCGAATGTC 3′
Aggrecan	Forward	5′ AGGCAGCGTGATCCTTACC 3′	136
	Reverse	5′ GGCCTCTCCAGTCTCATTCTC 3′
TIMP-3	Forward	5′ CCAGGACGCCTTCTGCAAC 3′	71
	Reverse	5′ CCTCCTTTACCAGCTTCTTCCC 3′
MMP-1	Forward	5′ GGGAGATCATCGGGACAACTC 3′	72
	Reverse	5′ GGGCCTGGTTGAAAAGCAT 3′
MMP-3	Forward	5′ TGGCATTCAGTCCCTCTATGG 3′	116
	Reverse	5′ AGGACAAAGCAGGATCACAGTT 3′
MMP-13	Forward	5′ AAGGAGCATGGCGACTTCT 3′	72
	Reverse	5′ TGGCCCAGGAGGAAAAGC 3′
ADAMTS-4	Forward	5′ CAAGGTCCCATGTGCAACGT 3′	115
	Reverse	5′ CATCTGCCACCACCAGTGTCT 3′
ADAMTS-5	Forward	5′ TGGCTCACGAAATCGGACA 3′	74
	Reverse	5′ GGAACCAAAGGTCTCTTCACAGA 3′
B2M	Forward	5′ GACTTGTCTTTCAGCAAGGA 3′	106
	Reverse	5′ ACAAAGTCACATGGTTCACA 3′
GAPDH	Forward	5′ ACAACTTTGGTATCGTGGAA 3′	458
	Reverse	5′ AAATTCGTTGTCATACCAGG 3′