. Author manuscript; available in PMC: 2016 Jul 13.

Published in final edited form as: J Cell Biochem. 2015 Sep 29;117(3):684–693. doi: 10.1002/jcb.25353

TABLE III.

Relative mRNA Expression of hMSC and hHT During Co-Culture

	A. hMSC
	Donor set 1		Donor set 2		Donor set 3		Donor set 4
Gene	Day 3	Day 7	Day 3	Day 7	Day 7	Day 14	Day 7	Day 14
Collagen I	1.01 ± 0.15	1.11 ± 0.13	1.1 ± 0.24	1.6 ± 0.27	1.1 ± 0.21	0.9 ± 0.48	1.0 ± 0.19	0.7 ± 0.16
Collagen III	1.05 ± 0.06	1.2 ± 0.30	1.7 ± 0.66	1.4 ± 0.49	1.0 ± 0.17	15 ± 032	0.9 ±0.12	1.0 ± 0.32
Tenascin C	0.49 ± 0.07^%	1.03 ± 0.30	10 ± 0.4	1.2 ± 0.21	0.90 ± 0.19	1.4 ± 039	0.5 ± 0.22	0.7 ± 0.40
Tenomodulin	ND	ND	5.2 ± 1.96^*	4.9 ± 20^*	1.0 ± 0.26	0.6 ± 020	4.2 ± 1.75^*	2.2 ± 0.20^*
Aggrecan	0.95 ± 1.13	0.80 ± 0.09	1.4 ± 0.25	2.5 ± 0.72^*	1.0 ± 0.39	0.7 ± 037	1.5 ± 0.15^*	3.8 ± 1.13^*
TIMP-3	1.29 ± 0.87	0.77 ± 0.2I	0.9 ± 0.21	1.5 ± 0.38	1.4 ± 0.22	0.8 ± 032	0.8 ± 0.05	0.9 ± 0.16
MMP-1	ND	ND	1.4 ± 0.16	1.0 ± 0.45	2.6 ± 0.11^*	1.7 ± 0.47	1.7 ± 1.12	0.9 ± 0.15
MMP-3	ND	ND	1.8 ± 0.70	0.8 ± 0.20	1.7 ± 0.42	12 ± 032	1.5 ± 0.55	0.8 ± 0.11
MMP-13	ND	0.85 ± 0.28	1.0 ± 0.62	1.1 ± 0.47	2.0 ± 0.39^*	05 ± 034	0.8 ± 0.14	0.8 ± 0.14
ADAMTS-4	1.20 ± 0.24	1.02 ± 0.03	1.0 ± 0.39	1.7 ± 0.58	1.7 ± 0.23	1.0 ± 039	1.0 ± 0.14	0.8 ± 0.25
ADAMTS-5	1.07 ± 0.08	1.19 ± 0.52	1.7 ± 0.85	3.0 ± 1.21^*	1.8 ± 0.35^*	1.1 ± 039	1.6 ± 0.29^*	1.5 ± 0.30

B. hHT

Collagen I	0.75 ± 0.11^*	l.03 ± 0.28	1.19 ± 0.25	1.39 ± 0.52	1.33 ± 0.44	3.95 ± 1.49^*	1.50 ± 0.54	0.91 ± 0.27
Collagen III	0.67 ± 0.09	1.11 ± 0.30	1.75 ± 0.46	1.23 ± 0.32	1.41 ± 0.54	1.63 ± 0.64^*	1.51 ± 0.08	1.07 ± 0.64
Tenascin C	l.32 ± 0.48	1.14 ± 0.33	2.19 ± 0.96	1.87 ± 0.35^*	0.95 ± 0.41	1.89 ± 1.18	2.09 ± 0.84	2.05 ± 0.35
Tenomodulin	ND	ND	ND	ND	ND	ND	1.39 ± 0.90	2.80 ± 1.52
Aggrecan	2.33 ± 1.90	0.84 ± 0.08	1.24 ± 0.40	1.04 ± 0.42	0.77 ± 039	0.62 ± 0.23	1.47 ± 0.43	0.74 ± 0.46
TIMP-3	2.12 ± 1.93	0.94 ± 0.33	1.85 ± 1.12	0.95 ± 0.20	2.06 ± 0.43^*	2.66 ± 0.64^*	2.27 ± 0.82	0.74 ± 0.30
MMP-1	4.32 ± 0.39^#	10.01 ± 4.73^*	0.65 ± 0.62	1.11 ± 0.08	1.16 ± 020	3.72 ± 0.77^%	4.14 ± 0.94^*	0.61 ± 0.30
MMP-3	ND	3.51 ± 1.34^*	1.37 ± 0.92	1.14 ± 0.14	1.90 ± 022^*	0.90 ± 0.23	2.06 ± 0.67	0.94 ± 0.45
MMP-13	8.07 ± 0.71^#	16.43 ± 5.15^%	1.0 ± 0.62	1.33 ± 0.26	1.05 ± 0.14	2.12 ± 0.29^%	3.16 ± 0.98^*	1.61 ± 0.11
ADAMTS-4	1.29 ± 0.45	3.08 ± 1.04^*	2.53 ± 0.94	1.43 ± 0.21	1.61 ± 0.19^*	2.03 ± 0.41^*	2.47 ± 1.01	0.98 ± 0.42
ADAMTS-5	0.44 ± 0.15	0.53 ± 0.25	0.54 ± 0.44	0.90 ± 0.24	0.64 ± 0.17	120 ± 0.37	1.18 ± 0.46	0.73 ± 0.29

Transcript levels expressed as relative compared to non co-cultured control cell populations. Normalized to B2M or GAPDH.

P < 0.05,

P < 0.01,

P < 0.001.