Skip to main content
. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cytokine. 2016 May 30;84:74–87. doi: 10.1016/j.cyto.2016.05.014

Figure 2. Regulation of apoptosis by butyrate in HL-60, THP-1 leukemia cells, and monocytes.

Figure 2

Figure 2

(A) HL-60, (B) THP-1 leukemia cells, and (C) monocytes isolated from whole blood taken from anonymous donors were treated with butyrate for 24 hours. Cell viability was determined using trypan blue exclusion assay. n = 6, 5, and 9 respectively. (D) Cells were untreated or treated with 5 or 10 mM butyrate for 24 hours and subjected to colorimetric caspase-3 assay. n = 6, 5, and 5 respectively. (E) HL-60 and (F) THP-1 cells were treated with butyrate (5 mM) and Annexin V fluorescence was measured by flow cytometry. n = 7. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 when compared to the untreated (0).