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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Cytokine. 2016 May 30;84:74–87. doi: 10.1016/j.cyto.2016.05.014

Figure 8. p38 MAPK does not regulate the production of CCL2 and CCL5 in U937 cells.

Figure 8

Figure 8

(A) Cells were untreated or pre-treated for 30 minutes with p38 MAPK inhibitor SB203580 (SB), p38 inducer Anisomycin, DMSO vehicle, and/or butyrate for 30 minutes or 24 hours. After harvesting and lysing the cells, Western blot analysis was carried out to analyze multiple proteins. Quantitative analyses of p-p38 and total p38 proteins were determined using densitometry. n = 5. (B) Cells were untreated or pre treated for 30 minutes with p38 MAPK inhibitor SB203580 (SB) followed with treatment of 5 mM butyrate for 6, 12, or 24 hours and analyzed for CCL2 and (C) CCL5 via ELISA. n = 5. **p < 0.01 when compared to the untreated (UT, 0).