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| Clone | Primer sequence (5’-3’) |
|---|---|
| pET21a-γS |
F: GGGAGTTCCATATGTCTAAAACTGGAACC |
| |
R: CCGGAATTCTTACTCCACAATGCG |
| pET21a-γSG57W |
F: GAAAGGCCCAACTTTGCTTGGTACATGTACATCTTACCACAGGGAG |
| |
R: GGTAAGATGTACATGTACCAAGCAAAGTTGGGCCTTTCATAAACAGCCC |
| T7 |
F: TAATACGACTCACTATAGG |
| T7 |
R: TATGCTAGTTATTGCTCAG |
| BGH | R: TAGAAGGCACAGTCGAGG |
