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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: ACS Chem Biol. 2015 Nov 6;11(1):121–131. doi: 10.1021/acschembio.5b00847

Figure 4.

Figure 4

A bivalent inhibitor of Abl. (A) (Top panel) SNAPtag-HA4 fusion that was generated for this study. GT = glycine-threonine. The exact sequence for this construct is shown in SI, Figure S3. (Bottom panel) The binding sites for the two ligands of the bivalent inhibitor are superimposed on a crystal structure of Abl3D, PDB ID 2FO0. (B) In vitro activities of 4, SNAPtag proteins, and assembled SNAPtag-4 conjugates against two different Abl constructs. Abl3D contains the kinase, SH2, and SH3 domains, while AblKD is the kinase domain alone. Values shown are the average of assays performed in triplicate ± SEM.