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. 2016 Jul 14;7:1112. doi: 10.3389/fmicb.2016.01112

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Copyright © 2016 Miernikiewicz, Kłopot, Soluch, Szkuta, Kęska, Hodyra-Stefaniak, Konopka, Nowak, Lecion, Kaźmierczak, Majewska, Harhala, Górski and Dąbrowska.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE of: (A) expressed recombinant His-tagged gp12 and gp57; overexpressed proteins are marked with arrows; M-molecular weight marker; 1-soluble fraction of the culture before induction (control); 2-soluble fraction of the culture after induction (expression); 3-insoluble fraction of the culture before induction (control); 4-insoluble fraction of the culture after induction (expression). (B) gp12 final preparation as trimeric and monomeric; M – molecular weight marker; 1 – heated gp12 (denatured protein) as monomeric (57.5 kDa); 2 – non-heated gp12 as trimeric (172.5 kDa).