Table 1.

Genes, whose expression is up-or down-regulated more than 10-fold (p < 0.01) in the liver of both non-vaccinated (N) and vaccinated mice (V) infected with P. chabaudi on day 11 p.i. (Vd11, Nd11) in comparison to constitutive expression on day 0 p.i. (Vd0, Nd0).

Gene	Gene description	RefSeq ID Agilent ID	Vd11 vs Vd0.	p-value	Nd11 vs Nd0.	p-value	Function (according to www.genecards.org)
UP-REGULATED GENES
Apol10b	Apolipoprotein L 10B	NM_177820 A_66_P120380	14	0.006	33	<0.001	“The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.”
Dlgap5	Discs, large (Drosophila) homolog-associated protein 5	NM_144553 A_55_P2050439	10	0.004	13	0.006	“Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.”
Fcho1	FCH domain only 1	NM_028715 A_55_P2047461	12	0.004	10	0.007	“Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors.”
Hbq1b	Hemoglobin, theta 1B	NM_001033981 A_52_P87503	13	0.008	23	0.005	“Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other non-erythroid tissue.”
DOWN-REGULATED GENES
Cd209b	CD209b antigen	NM_001037800 A_52_P267717	0.09	0.006	0.06	0.002	“Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.”
Cyp51	Cytochrome P450, family 51	NM_020010 A_52_P164161	0.09	0.003	0.09	<0.001	Phase I metabolism.
Cyp8b1	Cytochrome P450, family 8, subfamily b, polypeptide 1	NM_010012 A_51_P266618	0.06	<0.001	0.07	0.009	“Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid.”
Hmgcs2	3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2	NM_008256 A_55_P1992582	0.09	0.007	0.01	<0.001	“This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.”
Idi1	Isopentenyl-diphosphate delta isomerase	NM_145360 A_55_P2028961	0.08	0.001	0.04	<0.001	“IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol.”
Mup17	Major urinary protein 17	NM_001200006 A_55_P2076196	0.02	0.007	<0.01	0.001	“Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s).”
Mup19	Major urinary protein 19	NM_001135127 A_55_P1974080	0.03	<0.001	0.01	0.002	“Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s).”
Mup2	Major urinary protein 2	NM_001045550 A_55_P2010097	<0.01	0.004	<0.01	0.005	“Binds pheromones, likely to displace pheromones complexed to urinary MUPs and transport them to the vomeronasal organ (VNO) where they associate with their neuronal receptor(s).”
Ncmap	Noncompact myelin associated protein	NM_001243306 A_55_P2148171	0.07	0.004	0.05	0.009	“Plays a role in myelin formation.”
Prkcz	Protein kinase C, zeta	NM_008860 A_52_P284889	0.09	<0.001	0.08	0.007	“Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine.”
Sc4mol	Sterol-C4-methyl oxidase-like	NM_025436 A_51_P209372	0.08	<0.001	0.08	<0.001	“The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis.”
Serpina6	Serine (or cysteine) peptidase inhibitor, clade A, member 6	NM_007618 A_51_P133562	0.08	0.006	<0.01	0.007	“This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates.”
Slc22a7	Solute carrier family 22 (organic anion transporter), member 7	NM_144856 A_51_P395856	0.04	0.001	<0.01	0.005	“Mediates sodium-independent multispecific organic anion transport. Transport of prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate.”
Slc2a5	Solute carrier family 2 (facilitated glucose transporter), member 5	NM_019741 A_51_P514405	0.05	0.002	0.01	0.007	“Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.”