Fig. 7.
Immunolocalization of adenosine A2A (a–e) and A2B (f–j) receptors with Ezrin staining in Capan-1 monolayers. Fluorescence images of adenosine A2A receptors on the basolateral (a) and luminal (b) membranes in the Capan-1 monolayer. c Z-scan image of the same sample in a and b. d Fluorescence image of Ezrin. e Overlay image of c and d. Broken lines indicate the position of the permeable membrane. Fluorescence images of adenosine A2B receptors on the basolateral (f) and luminal (g) membranes in the Capan-1 monolayer. Z-scan images of adenosine A2B receptors (h), Ezrin (i), and overlay (j). DAPI was used to stain nuclei (blue). Bars = 20 μm