Fig. 4.

Representative images of teratogenic effects observed at 72 hpf following exposure of zebrafish to individual compounds, PAH mixture and sediment extracts from sampling regions. Exposures were as follows: 0.05 % DMSO control (a), 50 μM naphthalene (b), 50 μM phenanthrene (c), 2.5 μM pyrene (d), 25 μM B[a]P (e), 50 μM PAH mixture (f), water control (g), 0.25 % DMSO control (h), 25 mg Ilaje eQsed/mL (i), 6.25 mg Iddo eQsed/mL (j), 25 mg Atlas Cove eQsed/mL (k) and 2.5 mg Apapa eQsed/mL (l). Examples of defects: S scoliosis, SG stunted growth, PO pericardial oedema, YSO yolk-sac oedema, EH elongated heart, TTC tail tip curvature