Table 2.

Recorded abnormal developmental endpoints at 24, 48 and 72 hpf in zebrafish embryos

Endpoints	Description
Lethal
Coagulation	Embryo coagulated with no structures
Heartbeats	Embryo has no visible heartbeat
Teratogenic/developmental
Tail development	Tail is shorter than normal or curved and/or tail tip is malformed
Oedema	Oedema (swelling caused by fluid retention) is present in the yolk sac, pericardial region or both regions
Heart rate	Alterations to number of heart beats per minute (NHBpM)
Cardiac morphology
Haemorrhage	Visualized as a pool of blood in a tissue or organ
Thrombosis	Observed as a stagnant blood flow or blood clot in the cardinal vein
Scoliosis	Abnormal curvature of the spine to the side

Adapted from Kumar et al. (2013)