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J774 macrophages stimulated with Lp62 and/or LPS: J774 macrophages were stimulated with LPS and Lp62 for 2 hours. The levels of IL-1, TNF-α, and IL-17 were measured in culture supernatant by ELISA ((a), (b), and (c), resp.). The CD86 expression was analyzed by flow cytometry (d). ASignificant difference compared to unstimulated cells (culture medium). BSignificant difference from the control stimulated with LPS only; P < 0.05.
