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. 2016 Jul 14;6:29205. doi: 10.1038/srep29205

Table 1. Primers used in PCR.

Primer name (5′ → 3′) nucleotide sequence
GRK2-F1- EcoRI tactcagaattcaaaaagattgttctgcca
GRK2-R1-Xho I tactcactcgagtcagccgatgatgcggtg
GRK2-RNAi-F gcgtaatacgactcactatagggcgactttagcgtgcaccg
GRK2-RNAi-R gcgtaatacgactcactatagggtagcccgtgccttttgac
GFP-RNAi-F gcgtaatacgactcactataggtggtcccaattctcgtggaac
GFP-RNAi-R gcgtaatacgactcactataggagctggagacaactcctcacg
GRK2-G/YFP-F tactcacaattggatggcggacctcgaggct
GRK2-G/YFP-R tactcaggcgcgccgagttgctcccgttgggcgc
GRK2-T672A-GFP-F atctacggcgcggacggcgccaa
GRK2-T672A-GFP-R ttggcgccgtccgcgccgtagat
GRK2-S680A-GFP-F aaggacgccatggcgctggtgcg
GRK2-S680A-GFP-R cgcaccagcgccatggcgtcctt
GPCR-2-C/YFP-F tactcagagctcatgattacattcataacagtg
GPCR-2-C/YFP-R tactcacaattgggaggctgtttgatgttgagcga
GRK2-QRT-F aaagtgacaaatacacgaggtt
GRK2-QRT-F cttggacctgatgaacgg
Actin-QRT-F cctggtattctgaccgtatgc
Actin-QRT-R ctgttggaaggtggagagggaa
EcR-B1-QRT-F aattgcccgtcagtacga
EcR-B1-QRT-R tgagcttctcattgagga
USP1-QRT-F ggtcctgacagcaatgtt
USP1-QRT-R ttccagctccagctgactgaag
HR3-QRT-F tcaagcacctcaacagcagcccta
HR3-QRT-R gactttgctgatgtcaccctccgc
Br-QRT-F ggtgactgtccttactgcggcat
Br-QRT-R ttaattcctttgaccatgact
Kr-h1-F gcggtgggcctccacgtgtcgaacg
Kr-h1-R cgttcgacacgtggaggcccaccgc