Figure 3. Amino acid enrichment analysis of persulfide containing peptides and proteomic comparison of persulfides, S-nitrosated cysteines and S-sulfenylated cysteines.

(a) pLogo²⁹ analysis of enriched amino acids up to 15 positions before or after the reactive cysteine. Red: negative charged amino acids, blue: positive charged amino acids, black: hydrophobic amino acids (b) pLogo analysis of enriched amino acids 15 positions before or after cysteines that were not affected by H₂S stimulation found in the beads fraction. (c) Venn diagram comparing proteins that were S-sulfhydrated (R-S-SH, persulfides) by GYY4137 and Na₂S₄ as well as S-sulfenylated (R-S-OH) and S-nitrosated (R-S-NO). (d) Venn diagram comparing S-sulfhydrated (R-S-SH, persulfides), S-sulfenylated (R-S-OH) and S-nitrosated peptides (R-S-NO).