Skip to main content
. 2016 Jul 14;6:29668. doi: 10.1038/srep29668

Figure 2. Isolation of Arabidopsis acr11 and acr12 mutants.

Figure 2

(a) Positions of T-DNA insertions corresponding to acr11-1, acr11-2, acr12-1, and acr12-2. (b) RT-PCR analysis of ACR11, ACR12, Glu1, and PP2AA3 (internal standard) gene transcript levels in aboveground tissues of wild-type (WT) and mutant plants. (c) Soluble proteins extracted from rosette leaves of WT and mutant plants grown for 4 weeks were separated by SDS-PAGE on 6% (for Fd-GOGAT) or 14% (for ACR11) separation gels. ACR11 and Fd-GOGAT proteins were semi-quantified by immunoblot analysis using specific antibodies. 100% in the WT represents equal loading of proteins with acr11 and acr12 mutants, whereas 50%, 25%, and 12.5% represent loading amounts relative to the WT. (d) Visible phenotypes of acr11 and acr12 mutants grown for 4 weeks. Typically, acr11 mutants showed retarded growth and chlorotic spots on leaves, whereas acr12 mutants had no visible phenotypes. (e) Rosette diameters of wild-type and mutant plants grown for 4 weeks. Asterisks indicate significant differences from wild-type plants (one-way ANOVA, Dunnett’s test, P < 0.001). (f) Fd-GOGAT activities of rosette leaves of wild-type and acr 11-1 plants grown for 4 weeks. Asterisks indicate significant differences (Student’s t-test, P < 0.001).