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. 2016 Jul 14;6:29668. doi: 10.1038/srep29668

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(a) Soluble protein complexes from rosette leaves of wild-type, acr11, and acr12 plants grown for 4 weeks were separated by BN-PAGE and visualized by Coomassie Brilliant Blue staining. The putative Fd-GOGAT-ACR11 protein complex is indicated by an asterisk. (b,c) Immunoblot analysis of protein complexes separated by BN-PAGE with anti-Fd-GOGAT (b) and anti-ACR11 (c) antibodies. (d) Co-immunoprecipitation of Fd-GOGAT and ACR11-FLAG proteins with anti-FLAG antibody-conjugated agarose. Input (Input) and co-precipitated (Co-IP) proteins were separated by SDS-PAGE, followed by immunoblot analysis. Input represents the total leaf extract used for co-immunoprecipitation, whereas Co-IP represents co-precipitated proteins with anti-FLAG antibody-conjugated agarose.