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. 2016 Jul 14;6:29665. doi: 10.1038/srep29665

Figure 4. MAVS, TRIF and the downstream adaptor TBK1 upregulates SAMHD1 expression.

Figure 4

(A) HeLa cells, HEK293 cells and MARC-145 cells were transfected with MAVS or TRIF expression plasmids and analyzed using Western blotting. (B) TBK1 activation upregulates SAMHD1 expression and promoter luciferase activity. SAMHD1 promoter luciferase activity was measured in HeLa cells. Cells were transfected with TBK1 or empty vector for 24 h and luciferase reporter activity was measured. Results are expressed as the fold-increase of luciferase activity in TBK1 overexpression cells. The error bars represent standard deviation from three independent experiments and asterisks indicate a significant difference (**p < 0.01), compared to empty vector transfection. Western blotting analysis of SAMHD1 expression in HeLa cells (C), HEK293 cells (D) and MARC-145 cells (E) transfected with FLAG-tagged TBK1 WT and empty vector, respectively. The results are representative of three independent experiments. Expression levels of SAMHD1 compared to β-actin are shown. Uncropped images of blots are shown in Supplementary Figure 4.