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. 2016 Jul 14;6:29822. doi: 10.1038/srep29822

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Copyright © 2016, Macmillan Publishers Limited

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TRIM28 overexpression increases TWIST1 protein levels in 4T1 cells (B,C) and in HeLa cells (D,E). (A) TRIM28 protein expression by Western blotting in MCF7, T47D, BT549, MDA-MB-231(MDA231), MDA-MB-435(MDA435), 4T1 and HeLa cell lines. (B) Protein levels by Western blotting in 4T1 cells. (C) mRNA levels of TWIST1 in 4T1 cells. TWIST1 protein increases by 1.71 fold but mRNA do not increased (actually decreased) when overexpression of TRIM28. (D) TWIST1 protein levels by Western blotting in HeLa cells. (E) mRNA levels of TWIST1 by real-time PCR in HeLa cells. TWIST1 protein increases by 1.43 fold but mRNA did not increase (actually decreased) when overexpression of TRIM28 in HeLa cells. **P-value ≤ 0.05.