Figure 3. Comparison of mouse gut microbiota compositions between two sequencing platforms at deeper classifications (order to species).

(A) Heat map for the mean relative abundances for the two platforms at the order, family, genus, and species levels. Nanopore and Illumina columns are colored with relative abundances ranging from 0% to 100% according to the color key in the lower left corner of the figure. The +/− column is colored with log₂ fold-changes ranging from −2 to 2 according to the color key in the lower left corner of the figure. The black border indicates significantly different abundance deviation (log₂ fold-change, lower than −1 or higher than 1). The taxonomic units were categorized as I, II, and III according to whether they were detected by Illumina only (I), nanopore only (II), or both platforms (III). The Spearman rank correlation test (R²) showed the significance of relationships between the two platforms. Asterisks indicate the significance of the pairing (*p < 0.05, ****p < 0.0001). (B) Proportion of groups (I–III) at the levels of taxonomic classification from order to species.