FIG 1.

CrhR abundance in cytoplasmic and membrane fractions. Wild-type (WT) and crhR_TR mutant strains were grown at 30°C (A), and half of the culture was incubated at 20°C for 3 h (B). Cells were mechanically lysed and clarified by centrifugation at 13,000 × g to generate crude soluble cytoplasmic (lanes C) and membrane (lanes M) fractions. Soluble protein (10 μg) or an equivalent volume of the membrane fraction was subjected to Western analysis. CrhR (55 kDa), S1 (40 kDa), and CrhR_TR (27 kDa) were detected using anti-CrhR and anti-S1 antibodies and ECL.