TABLE 1.
CrhR and PsbA ratio between thylakoid membrane region and cytoplasm of immunogold-labeled wild-type and crhRTR Synechocystis cellsa
| Protein and cell type | Result for: |
|||
|---|---|---|---|---|
| 20°C |
30°C |
|||
| No. of gold particles | TM/C ratio ± SD | No. of gold particles | TM/C ratio ± SD | |
| CrhR | ||||
| Wild type | 442 | 1.64 ± 0.22 | 264 | 1.54 ± 0.25 |
| crhRTR | 1,367 | 1.44 ± 0.24 | 200 | 0.70 ± 0.19 |
| PsbA (D1) | ||||
| Wild type | 372 | 16.36 ± 11.7 | 107 | 10.18 ± 1.14 |
| crhRTR | 126 | 21.46 ± 9.65 | 250 | 15.34 ± 6.67 |
a
Warm-grown (30°C) and cold-shocked (20°C for 3 h) cells were viewed using immunoelectron microscopy. Gold particles were enumerated manually from the digital images obtained from ultrathin sections. Normalization was performed by accounting for the area of the thylakoid membrane region (TM), the cytoplasm (C), and the surrounding resin background.