TABLE 1.
Sporulation efficiencies of yodL and yisK mutantsa
| Genotype | Strain | Total CFU | Heat-resistant CFU | % sporulation efficiency | % relative sporulation efficiency |
|---|---|---|---|---|---|
| Wild type | B. subtilis 168 | 2.8 × 108 (4.7 × 107) | 1.9 × 108 (4.5 × 107) | 66.9 (5) | 100 |
| ΔyodL | BYD276 | 2.6 × 108 (3.9 × 107) | 1.7 × 108 (2.8 × 107) | 65.2 (7) | 97 |
| ΔyisK | BYD278 | 2.7 × 108 (4.6 × 107) | 2.4 × 108 (2.7 × 107) | 63.1 (6) | 94 |
| ΔyodL ΔyisK | BYD279 | 3.1 × 108 (6.5 × 107) | 1.7 × 108 (4.1 × 107) | 54.1 (4) | 81 |
| ΔyodL ΔyisK PyisK-yisK | BYD510 | 3.4 × 108 (3.3 × 107) | 2.3 × 108 (4.1 × 107) | 66.2 (7) | 99 |
a
Sporulation efficiency was calculated as the number of spores per milliliter, divided by the total CFU per milliliter, times 100. Relative sporulation efficiency is the sporulation efficiency normalized to that of the wild type (times 100). Values are means, with standard deviations in parentheses. The data shown are the average results for six independent biological replicates. The difference in sporulation efficiency between the wild type and the ΔyodL ΔyisK double mutant was statistically significant (P < 0.0006).