FIG 3.
Involvement of SPases in sortase-associated GspA glycosylation. (A) Cells were grown to mid-log phase, and normalized by OD. Culture supernatant (S), cell wall (W), membrane (M), and cytoplasm (C) fractions were separated by SDS-PAGE and immunoblotted with antibodies to GspA and membrane protein MdbA, which served as loading and fractionation controls. Brackets designate glycosylated HMW species of GspA polymers (GspAP). (B) The relative expression levels of gspA in the strains indicated were determined by quantitative real-time PCR as described in the legend to Fig. 2. Error bars show the standard deviations of two independent experiments performed in triplicate.