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The SPase LepB2 is required for pilus assembly. A. oris cells were immobilized on nickel grids and reacted with antiserum specific to the type 2 major pilin subunit FimA (A to E) or the type 1 major pilin subunit FimP (F to J), followed by goat anti-rabbit IgG conjugated to 12-nm gold particles, and stained with 1.0% uranyl acetate. Samples were viewed by transmission EM. Scale bars, 0.2 μm.
