FIG 3.
Disruption of codY leads to increased sporulation frequency. Phase-contrast microscopy images of the 630Δerm, MC364 (630 codY), MC442 (630 codY Tn::codY), UK1, LB-CD16 (UK1 codY), and MC443 (UK1 codY Tn::codY) strains grown in 70:30 liquid sporulation medium at T24 are shown. Percentages of phase-bright spores were evaluated through direct counting of phase-contrast micrographs. Arrowheads indicate phase-bright spores. The means and SEM of spore counts from four biological replicates are shown. Sporulation frequencies of the parent strains were compared to those of the respective codY mutants and complemented strains using one-way ANOVA (*, P ≤ 0.05).