FIG 4.

Increased expression of sporulation-specific factors in codY mutants. Data represent results of qRT-PCR analysis of spo0A (A and B), sigF (C and D), sigE (E and F), and sigG (G and H) expression in the 630Δerm, MC364 (630 codY), and MC442 (630 codY Tn916::codY) strains (A, C, E, and G) and in the UK1, LB-CD16 (UK1 codY), and MC443 (UK1 codY Tn916::codY) strains (B, D, F, and H) grown in 70:30 sporulation medium. Samples for RNA isolation were collected during logarithmic growth (Log; OD₆₀₀, ∼0.5), during the transition to stationary phase (T₀), and 4 h after the transition into stationary phase (T₄). The means and SEM of results from four biological replicates are shown. Two-way repeated-measures ANOVA followed by Dunnett's multiple-comparison test was used to compare the parent strain with the codY mutant or the parent strain with the respective complemented strain at the designated time points. *, P ≤ 0.05.