FIG 5.

Expression of sinRI is differentially regulated by CodY. qRT-PCR expression analysis of sinR and sinI in strains 630Δerm, MC364 (630 codY), and MC442 (630 codY Tn916::codY) (A and C) and in strains UK1, LB-CD16 (UK1 codY), and MC443 (UK1 codY Tn916::codY) (B and D) grown in 70:30 sporulation medium. Samples for RNA isolation were taken during logarithmic growth (Log; OD₆₀₀ ∼0.5), during the transition to stationary phase (T₀), and 4 h after the transition into stationary phase (T₄). The means and SEM of results from at least four biological replicates are shown. Two-way repeated-measures ANOVA followed by Dunnett's multiple-comparison test was used to compare the parent strain with the codY mutant strain or the parent strain with the respective complemented strain at the designated time points. *, P ≤ 0.05.