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. 2016 Jul 13;198(15):2113–2130. doi: 10.1128/JB.00220-16

FIG 6.

FIG 6

Expression of the genes encoding the peptide permeases, opp and app. Data represent results of qRT-PCR expression analysis of oppB and appA in strains 630Δerm, MC364 (630 codY), and MC442 (630 codY Tn916::codY) (A and C) and strains UK1, LB-CD16 (UK1 codY), and MC443 (UK1 codY Tn916::codY) (B and D) grown in 70:30 sporulation medium. Samples for RNA isolation were taken at logarithmic growth (Log; OD600, ∼0.5), at the transition to stationary phase (T0), and 4 h after the transition into stationary phase (T4). The means and SEM of results from four biological replicates are shown. Two-way repeated-measures ANOVA followed by Dunnett's multiple-comparison test was used to compare the parent strain with the codY mutant or the parent strain with the respective complemented strain at the designated time points. *, P ≤ 0.05.