FIG 7.

Germination phenotypes of codY mutants. The germination of C. difficile heat-activated spores suspended in BHIS medium with or without 5 mM taurocholate (TA) was monitored over time. (A and B) The germination of strains 630Δerm, MC364 (630 codY), and MC442 (630 codY Tn916::codY) (A) or strains UK1, LB-CD16 (UK1 codY), and MC443 (UK1 codY Tn916::codY) (B) is shown as a ratio of the OD₆₀₀ at a given time point (t) to the OD₆₀₀ at the initial time point (t₀). The means and SEM of results from three biological replicates are shown. Where error bars are not visible, they are obscured by symbols. Two-way repeated-measures ANOVA followed by Dunnett's multiple-comparison test was used to compare the parent strain with the codY mutant or the parent strain with the respective complemented strain at the designated time points. *, P ≤ 0.05.