TABLE 4.
Alkaline phosphatase activity from the sinR promotera
| Promoter fusion or gene (strain background[s]) | Time point | Alkaline phosphatase activity (mean ± SEM) for genotype: |
|
|---|---|---|---|
| 630Δerm | 630 codY | ||
| PsinR600::phoZ (MC560/MC596) | Log | 7.2 ± 0.9 | 11.9 ± 1.1 |
| T4 | 7.0 ± 0.7 | 10.9 ± 1.5 | |
| PsinR600(C-290A)::phoZ (MC769) | Log | 8.6 ± 1.0 | NDb |
| T4 | 9.0 ± 1.0 | ND | |
| phoZ (MC448/MC589) | Log | 0.6 ± 0.1 | 0.8 ± 0.1 |
| T4 | 0.7 ± 0.1 | 0.9 ± 0.2 | |
a
Data were analyzed by two-way ANOVA followed by Tukey's test for multiple comparisons. Values are expressed in alkaline phosphatase activity units. Bold text indicates a P value of ≤0.05 for comparisons between data from the same promoter fusion at different time points or comparisons between data from the same promoter fusion with and without CodY. MC560 was run as a positive control for all experiments; n = 8. Log, logarithmic growth phase; T4, stationary phase.
b
ND, not determined.