TABLE 5.
Alkaline phosphatase activity of the opp and app promoter regions during logarithmic growtha
| Promoter fusion or gene | Strain background(s) | Promoter template | Alkaline phosphatase activity (mean ± SEM) for genotype: |
|
|---|---|---|---|---|
| 630Δerm | 630Δerm codY | |||
| PoppB400::phoZ | MC565/MC601 | 630Δerm | 244.5 ± 13.3 | 503.5 ± 39.9 |
| MC608/MC611 | UK1 | 271.5 ± 8.5 | 446.0 ± 13.5 | |
| PoppB400(G-181A)::phoZ | MC769 | 630Δerm | 142.2 ± 3.1 | NDb |
| PoppB250::phoZ | MC650/MC655 | 630Δerm | 245.1 ± 15.4 | 373.3 ± 41.0* |
| MC651/MC659 | UK1 | 287.6 ± 24.5 | 422.2 ± 22.4 | |
| PoppB170::phoZ | MC649/MC657 | 630Δerm | 101.7 ± 4.3 | 118.4 ± 11.1 |
| MC653 | UK1 | 107.1 ± 10.6 | ND | |
| PoppB150::phoZ | MC647/MC658 | 630Δerm | 1.6 ± 0.2 | 1.8 ± 0.1 |
| MC654/MC662 | UK1 | 1.1 ± 0.1 | 1.5 ± 0.1 | |
| PappA600::phoZ | MC572/MC576 | 630Δerm | 21.7 ± 3.5 | 16.6 ± 2.1 |
| MC574/MC578 | UK1 | 14.5 ± 2.2 | 12.4 ± 1.7 | |
| phoZ | MC448/MC589 | 0.6 ± 0.1 | 0.8 ± 0.1 | |
a
Data were analyzed by two-way ANOVA followed by Tukey's test for multiple comparisons. Values are expressed in alkaline phosphatase activity units. Bold text indicates a P value of ≤0.05 for the comparison of promoter activities between strains carrying the same fusion with and without CodY. Underlined text indicates a P value of ≤0.05 for the comparison of promoter activities from fusions of the same length in the same background strain. An asterisk indicates a P value of ≤0.05 for the comparison between PoppB400 and PoppB250 in the same strain background. MC565 was run as a positive control for all experiments; n = 8.
b
ND, not determined.