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. 2016 Jul 14;6:29714. doi: 10.1038/srep29714

Figure 2. In vitro TERTp functional assays in the cell lines BON, CM and QGP1.

Figure 2

(A) Normalized fold change in the reporter assays activity for the vectors mock, wild-type TERTp, −124 and −146 TERTp mutated vectors; Even though we only detected the −124 mutation in our samples, we also created a reporter for −146 mutation, the second most frequent alteration in other human cancers; (B) Quantitative analysis by RT-PCR of the ChIP revealed that QGP1 (TERTp mutated) cell line, presents significant higher amount of ETS transcription factors in comparison to BON and CM cell lines (TERTp wild-type). Additionally, to ELK1 and ELK4, we detected that GABP-α and ETV1, with the ability to bind to TERTp regions. The results are an average of at least three independent experiments. Significance levels: P < 0.0001, ****; 0.0001 < P < 0.001, ***; 0.001 < P < 0.01, **; 0.01 < P < 0.05; and P ≥ 0.05, *; and not significant (ns). Values are mean ± SEM.