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. 2016 Jul 11;90(15):6832–6845. doi: 10.1128/JVI.00352-16

FIG 8.

FIG 8

Characterization of the interactions between SKP2 and ISG12a/NS5A. (A) Schematic representation of the domains of SKP2. (B and C) IP analysis of the interaction between SKP2 and ISG12a in HEK293T cells expressing exogenous ISG12a and multiple domains of SKP2 (B), or exogenous SKP2 and the different domains of ISG12a (C) for 48 h. (D and E) IP analysis of the interaction between SKP2 and NS5A in HEK293T cells expressing exogenous SKP2 and multiple domains of NS5A (D) or exogenous NS5A and the different domains of SKP2 (E) for 48 h. V5 and Flag were the tags used. SKP2-N, N-terminal of SKP2. SKP2-(N+L), SKP2 truncation containing SKP2-N and LRR. SKP2-DL, SKP2 with LRR deletion.