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. 2016 Jul 11;90(15):6832–6845. doi: 10.1128/JVI.00352-16

TABLE 1.

Primers for amplification of SKP2 domains

Domain and primer orientation Primer sequence
pcDNA3.1a-SKP2-N
    Forward GGAATTCATGCACGTATTTAAAACTCC
    Reverse CCCAAGCTTCTTGGAACACTGAGACAG
pcDNA3.1a-SKP2-(N+L)
    Forward GGAATTCATGCACGTATTTAAAACTCC
    Reverse CCCAAGCTTGCAATTAATCTGTAGATGAG
pcDNA3.1a-SKP2-DL
    Forward CCGCTCGAGATGCACGTATTTAAAACTCC
    Reverse GGAATTCTCTTGGAACACTGAGACAGTATG
    Forward GGAATTCTCCCATTTCACCACCATTGC
    Reverse CCCAAGCTTTCATAGACAACTGGGCTTTTG
pcDNA3.1a-SKP2
    Forward GGAATTCATGCACGTATTTAAAACTCC
    Reverse CCCAAGCTTTCATAGACAACTGGGCTTTTG