TABLE 2.

Primers for amplification of ISG12a domains

Domain and primer orientation	Primer sequence
p3×Flag-ISG12a-DII
Forward	GGGGTACCATGGTGATTGGAGGAGTTGTGGC
Reverse	GCTCTAGAGTAGAACCTCGCAATGACAG
p3×Flag-ISG12a-DII-N
Forward	GGGGTACCATGGTGATTGGAGGAGTTGTGGC
Reverse	GCTCTAGAAACTCCACCCCCATTGGCAA
p3×Flag -ISG12a-DII-C
Forward	GGGGTACCATGGGTGGAGTTGCCTCGGGC
Reverse	GCTCTAGAGTAGAACCTCGCAATGACAG
p3×Flag-ISG12a-DII-(106–327)
Forward	GGGGTACCATGGTGATTGGAGGAGTTGTGGC
Reverse	GCTCTAGAAGACCCAATGGAGCCCAGGA
p3×Flag-ISG12a-DII-(106–297)
Forward	GGGGTACCATGGTGATTGGAGGAGTTGTGGC
Reverse	GCTCTAGACAATCCGGAGAGTCCAGTTG
p3×Flag-ISG12a-DII-(106–267)
Forward	GGGGTACCATGGTGATTGGAGGAGTTGTGGC
Reverse	GCTCTAGACTGCAGAGTAGCCACAAGGC
pcDNA3.1a-ISG12a
Forward	GGGGTACCATGGAGGCCT CTGCTCTCAC
Reverse	GCTCTAGAGTAGAACCTCGCAATGACAG