TABLE 1.

Names and sequences of the primers used for cloning and mutagenesis

Primer	Sensea	Sequence (5′ → 3′)b	Purposec
RT-S	Forw.	ACACAAAGACCTCCAACTTAGCTATCG	RT, S segment
RT-M	Forw.	ACACAAAGACGGCTAACATGGTAAGG	RT, M segment
RT-L	Forw.	ACACAAAGACGCCAAGATGCTTTTAGCG	RT, L segment
UUKV-S-5NC	Forw.	AATCGTCTCTAGGTACACAAAGACCTCCAACTTAGCTATCG	Cloning the S segment into pRF108 (pRF108-S)
UUKV-S-3NC	Rev.	AATCGTCTCTGGGACACAAAGACCCTCC
UUKV-M-5NC	Forw.	AATCGTCTCTAGGTACACAAAGACGGCTAACATGGTAAGG	Cloning the M segment into pRF108 (pRF108-M)
UUKV-M-3NC	Rev.	AATCGTCTCGGGGACACAAAGACACGGCTACATGG
UUKV-L-5NC	Forw.	AATCGTCTCTAGGTACACAAAGACGCCAAGATGCTTTTAGCG	Cloning the L segment into pRF108 (pRF108-L)
UUKV-L-3NC	Rev.	AATCGTCTCGGGGACACAAAGTCCGCCAAGATGGAAGTAAAGG
Mut-M-S	Forw.	CAAGGATTCAGTGGATTGTCAATCATCAATCATAGATCCCA	Mutagenesis of the M segment (G2386A)
Mut-M-AS	Rev.	TGGGATCTATGATTGATGATTGACAATCCACTGAATCCTTG

^aForw, forward; Rev, reverse.

^bThe virus RNA sequence that is targeted is in italics, and the sequences introduced for cloning are in roman type. Underlined nucleotide sequences indicate a BsmBI restriction site. Bold nucleotides are the point mutations introduced in the M segment sequence of the UUKV lab strain.

^cRT, reverse transcription.