TABLE 2.
Full-length sequence of the viral glycoproteins GN and GC obtained from UUKV-infected field Ixodes ricinus nymphal ticks
| Region (aa)a | Isolate | Identity (%)b | Positive sequence (%)c | Substitution(s) relative to the UUKV S23 polypeptide precursord |
|---|---|---|---|---|
| GN (18–496) | RVS | 98.5 | 99.6 | L8I, L29I, S124T, T167S, L207V, A219T, T237A |
| HRS | 97.6 | 98.7 | L29I, T44A, S124T, T167S, L207V, A219T, S282T, T287A, N476K, N477Q, A479C | |
| Intraregion | RVS | 100.0 | 100.0 | |
| (497–513) | HRS | 97.1 | 97.1 | A508T |
| GC (514–1008) | RVS | 99.6 | 100.0 | S695T, Q790R |
| HRS | 99.2 | 99.8 | K577R, Q790R, T841S, L1003F |
Amino acid (aa) position.
Percent identity relative to the sequence of the precursor peptide.
Values represent the percent identity of the sequence of interest augmented by the substitutions shown in boldface, which are assumed to have a weak impact, relative to the sequence of the precursor protein.
Total RNAs were extracted from 16 pools of 25 nymphs collected in the region of Ramsvik and Hindens Rev, Sweden, in 2013. The cDNA corresponding to the M segment of UUKV was synthesized by RT-PCR and amplified by a single PCR. Out of the 16 nymphal pools, four were positive for the virus, and from these four samples, one partial and two full-length nucleotide sequences could be obtained and. The list is shown of the amino acids found to be mutated in the full-length amino acid sequences of isolates RVS and HRS (GenBank accession numbers KX219593 and KX219594, respectively) relative to the sequence of the polypeptide precursor of the two glycoproteins GN and GC of UUKV S23 (GenBank accession number NC_005220.1). The point mutations in boldface indicate positive substitutions.