FIG 5.

Vaccinated CD47KO mice decrease immune cell recruitments upon influenza virus infection. Immune cells were harvested from the BALF, lungs, and MLN at 6 dpi with A/PR8/34 influenza virus. The frequencies and numbers of monocytes (A and B) and neutrophils (A and C) were analyzed at 6 dpi in the BALF and lungs in WT and CD47KO mice. (D) Respiratory DC subsets were analyzed in the MLN using specific markers, such as CD11c, CD11b, and MHC-II. The statistical significance was determined by using one-way ANOVA. Error bars indicate the means ± the SEM of the concentrations or ratios from individual animals. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, no significant difference.