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. 2016 Jun 7;28(6):1328–1342. doi: 10.1105/tpc.15.00774

Figure 5.

Figure 5.

Kinase Activation in Response to Abiotic Stress.

(A) In-gel kinase activity assay with MBP or synthetic peptide P3 as substrates, and immunoblot analysis of MPK phosphorylation with α-pTEpY of extracts from wild type treated with water, 100 μg/mL chitin (Chi), 500 mM NaCl, or 800 mM mannitol for indicated times. Loading control is Ponceau staining.

(B) In-gel kinase assay with MBP and immunoblotting of MPK phosphorylation with α-pTEpY of extracts of the wild type or the ABA-insensitive line (D2-1) treated with 100 μg/mL chitin (Chi), 500 mM NaCl, or 10 μM ABA. Loading control is Ponceau staining of total protein.

(C) In-gel kinase assay with MBP of extracts of the wild type and Δsnrk2a-1 untreated (N.T.) or treated with water, 500 mM NaCl, or 10 µM ABA for 10 min.

(D) Quantitation of kinase activities in extracts relative to the wild type of two Δsnrk2a lines treated with 500 mM NaCl for 10 min. Data points represent an average of three biological replicates with sd as error bars.