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. 2016 Jun 14;28(6):1388–1405. doi: 10.1105/tpc.16.00125

Figure 7.

Figure 7.

ABI5 Enhances PIF1 Binding to PBSs.

(A) Real-time single-molecule fluorescence imaging analysis shows that ABI5 enhances PIF1 binding to a PIL2 promoter fragment containing three G-boxes. Promoter fragments are immobilized on a slide and binding events are detected by TIRF as GFP molecules approach the surface. Error bars indicate se (n = 3 locations).

(B) The enhancement of PIF1 binding to the PIL2 promoter fragment by ABI5 requires intact G-boxes. PIL2pro indicates immobilized PIL2 promoter fragments containing three intact G-boxes, while mPIL2pro indicates the same fragment with three mutated G-boxes. Error bars indicate se (n = 3 locations).

(C) ABI5 enhances PIF1 binding to an At4g32300 promoter fragment containing a GCE2. Error bars indicate se (n = 3 locations).

(D) The enhancement of PIF1 binding to the At4g32300 promoter fragment by ABI5 requires an intact GCE2. GCE2 indicates immobilized promoter fragments containing an intact GCE2, while mGCE2 indicates the same fragments with a mutated GCE2. Error bars indicate se (n = 20 locations).